 Iskandar ME, Aslani A, Tian Q*, and Liu H. Nanostructured
calcium phosphate coatings on magnesium alloys: characterization and
cytocompatibility with mesenchymal stem cells. Journal of Materials Science: Materials in Medicine 26(5): 1-18, 2015. This article reports the deposition and characterization of
nanostructured calcium phosphate (nCaP) on magnesium–yttrium alloy
substrates and their cytocompatibility with bone marrow derived
mesenchymal stem cells (BMSCs). The nCaP coatings were deposited on
magnesium and magnesium–yttrium alloy substrates using proprietary
transonic particle acceleration process for the dual purposes of
modulating substrate degradation and BMSC adhesion. Surface morphology
and feature size were analyzed using scanning electron microscopy and
quantitative image analysis tools. Surface elemental compositions and
phases were analyzed using energy dispersive X-ray spectroscopy and
X-ray diffraction, respectively. The deposited nCaP coatings showed a
homogeneous particulate surface with the dominant feature size of
200–500 nm in the long axis and 100–300 nm in the short axis, and a Ca/P
atomic ratio of 1.5–1.6. Hydroxyapatite
was the major phase identified in the nCaP coatings. The modulatory
effects of nCaP coatings on the sample degradation and BMSC behaviors
were dependent on the substrate composition and surface conditions. The
direct culture of BMSCs in vitro indicated that multiple factors,
including surface composition and topography, and the
degradation-induced changes in media composition, influenced cell
adhesion directly on the sample surface, and indirect adhesion
surrounding the sample in the same culture. The alkaline pH, the
indicator of Mg degradation, played a role in BMSC adhesion and
morphology, but not the sole factor. Additional studies are necessary to
elucidate BMSC responses to each contributing factor. |
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